Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells

Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells

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There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications.Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay.Then the cell membrane integrity and P-glycoprotein expression SPEED ORANGE were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.

Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.This study indicates that the resistance Electrical to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.